详细描述:
Species predicted to react based on 100% sequence homology: Chicken, Xenopus。Phospho-Src Family (Tyr416) Antibody兔多抗能够检测内源性的Tyr416位点磷酸化的Src蛋白。该抗体可能会与相同位点被磷酸化的其他Src家族成员(Lyn, Fyn, Lck, Yes和Hck)发生交叉反应。该抗体不会与Tyr527位点被磷酸化的Src蛋白发生交叉反应。可能与磷酸化的RTKs发生交叉反应。该多克隆抗体是采用与人源Src蛋白Tyr416位点附近相一致的合成磷酸化肽段免疫动物而产生的。该抗体经蛋白A和肽亲和层析纯化。Phospho-Src Family (Tyr416) Antibody detects endogenous levels of Src only when phosphorylated at tyrosine 416. The antibody may cross-react with other Src family members (Lyn, Fyn, Lck, Yes and Hck) when phosphorylated at equivalent sites. It does not cross-react with Src phosphorylated at tyrosine 527. It may cross react with phosphorylated RTKs.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr416 of human Src. Antibodies are purified by protein A and peptide affinity chromatography.