详细描述:
Phospho-Mnk1 (Thr197/202) Antibody检测仅在苏氨酸197和202位点磷酸化的内源性Mnk1蛋白。该抗体不与磷酸化的Mnk2a和Mnk2b蛋白发生交叉反应。通过人工合成小鼠Mnk1蛋白Thr197和Thr202位点周围相应的磷酸化片段去免疫动物从而制备多克隆抗体。通过蛋白A和多肽亲和层析纯化抗体。Phospho-Mnk1 (Thr197/202) Antibody detects endogenous levels of Mnk1 only when phosphorylated at threonines 197 and 202. The antibody also cross-reacts with phosphorylated Mnk2a and Mnk2b.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Thr197 and Thr202 of mouse Mnk1. Antibodies are purified by protein A and peptide affinity chromatography.