详细描述：
Phospho-IRS-1 (Ser612)抗体识别内源性的Ser612磷酸化的鼠IRS-1或Ser616磷酸化的人IRS-1。此抗体不与其它部位磷酸化的IRS-1蛋白发生交叉反应。该多克隆抗体用与鼠IRS-1中Ser612位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化制得。
Phospho-IRS-1 (Ser612) Antibody detects endogenous IRS-1 only when phosphorylated at serine 612 of mouse IRS-1 or serine 616 of human IRS-1. The antibody does not detect IRS-1 phosphorylated at other sites. Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser612 of mouse IRS-1. Antibodies are purified by protein A and peptide affinity chromatography.
