详细描述:
The Luciferase Assay Kit provides a rapid, sensitive, and quantitative measurement of the activity of the reporter enzyme American firefly (Photinus pyralis) luciferase in cultured mammalian cells. The kit is designed for use with the Agilent PathDetect in vivo signal transduction pathway reporting systems and other systems that use firefly luciferase as a reporter enzyme in mammalian cells. The luciferase assay is highly sensitive—as few as ~1 × 10 –20 moles of luciferase (1 × 105 luciferase molecules) are detected with a luminometer under optimal conditions. Background activity is low because mammalian cells do not contain endogenous luciferase. For Research Use Only. Not for use in diagnostic procedures