详细描述:
The StrataClone PCR Cloning Kit allows high-efficiency, 5-minute cloning of PCR products at room temperature, using the efficient DNA rejoining activity of DNA topoisomerase I and the DNA recombination activity of Cre recombinase. These kits are available for both blunt-end and UA cloning. Additionally, we have combined the highest accuracy in PCR amplification with easy, reliable, and affordably priced topoisomerase-based cloning in our StrataClone Ultra PCR Cloning System, which includes the PfuUltra II Fusion Hotstart DNA Polymerase. High efficiency yields >95% insert positive clones Clone both long and short amplicons (500 bp – 9.2 Kb) with the same kit With Kanamycin and Ampicillin Resistance Markers For Research Use Only. Not for Use in Diagnostic Procedures.