详细描述：
Random oligonucleotides have been used as primers in a method for labeling DNA to produce high specific-activity probes.1,2 The procedure relies on the ability of random hexanucleotides to anneal to multiple sites along the length of a DNA template. The primer–template complexes formed represent a substrate for the Klenow fragment of DNA polymerase I. The enzyme synthesizes new DNA by incorporating nucleotide monophosphates at the free 3´–OH group provided by the primer. The newly synthesized DNA is made radioactive by substituting a radiolabeled nucleotide for a nonradioactive one in the reaction mixture. The resulting labeled DNA can serve as a sensitive hybridization probe for screening gene libraries,3 probing Southern and northern blots,4,5 and in situ hybridization techniques.
