详细描述：
The Prime It RmT random primer labeling kit uses random oligonucleotides as primers for labeling DNA to produce high-specific-activity probes.The procedure relies on the ability of random 9-mers to anneal to multiple sites along the length of a DNA template. The primer–template complexes formed represent a substrate for the magenta DNA polymerase, a thermostable polymerase that allows the Stratagene Prime-It RmT random primer labeling kit to be stored at room temperature. The enzyme synthesizes new DNA by incorporating nucleotide monophosphates at the free 3´-OH group provided by the primer. The newly synthesized DNA is made radioactive by substituting radiolabeled [α-32P]dCTP for unlabeled dCTP in the reaction mixture. The resulting labeled DNA serves as a sensitive hybridization probe for screening gene libraries,4 probing Southern and Northern blots,5,6 and in situ hybridization techniques.
