Anti-rabbit IgG F(ab')2 Fragment (Sepharose® Bead Conjugate)
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详细描述：
免疫电泳后出现一条针对抗羊血清、兔IgG和兔血清的沉淀弧，没有出现针对抗胃蛋白酶、抗羊IgGFc或人或小鼠血清蛋白的反应。此种抗体经初级氨基酸团与N-基琥珀酰亚胺酯活化的琼脂糖微球共价结合固定。 Anti-Rabbit IgG F(ab’)2 Fragment (结合琼脂糖微球) 用于兔源抗体的免疫沉淀。该试剂是用兔源IgG全分子免疫羊生产的， Anti-Rabbit IgG F(ab’)2 Fragment 是通过采用兔源IgG偶联琼脂糖微球的免疫亲和色谱法中的单一抗血清制备的，然后采用固相吸附去除不必要的反应如胃蛋白酶消化和色谱分离。Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-goat serum, Rabbit IgG and Rabbit serum. No reaction was observed against anti-pepsin, anti-goat IgG Fc, or human or mouse serum proteins. This Cell Signaling Technology antibody is immobilized via covalent binding of primary amino groups to N-hydroxysuccinimide (NHS)-activated sepharose beads. Anti-rabbit IgG F(ab') 2 Fragment (Sepharose Bead Conjugate) is useful for the immunoprecipitation of antibodies raised in rabbit. Anti-rabbit IgG F(ab')2 Fragment is produced by immunizing goats with rabbit IgG whole molecules. The Anti-rabbit IgG F(ab')2 Fragment is prepared from monospecific antiserum by immunoaffinity chromatography using Rabbit IgG coupled to agarose beads followed by solid phase adsorption to remove any unwanted reactivities, pepsin digestion, and chromatographic separation.
