详细描述：
Phospho-PKC (pan) (βII Ser660)抗体可识别内源性的与PKCβII Ser660磷酸化同源磷酸化的PKC α、βI、βII、δ、ε、η、θ亚型。此抗体不与其它部位磷酸化的PKC蛋白发生交叉反应。该多克隆抗体用与人类PKCβII中Ser660位点附近的氨基酸序列对应的人工合成磷酸化肽段免疫动物而制成。该抗体使用蛋白A和蛋白亲和层析纯化而得。Phospho-PKC (pan) (βII Ser660) Antibody detects endogenous levels of PKC alphaα, β I, β II, δ, ε, η and θ isoforms only when phosphorylated at a carboxy-terminal residue homologous to serine 660 of PKC β II. This antibody does not detect PKC phosphorylated at other sites.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser660 of human PKC β II. Antibodies are purified by protein A and peptide affinity chromatography.
