详细描述：
TNP半抗原和荧光素报道基团与氨基乙基羧基甲基-聚蔗糖（AECM-FICOLL，一种高分子量多糖）偶联。 半抗原-聚蔗糖偶联物可用作研究B淋巴细胞活化的T非依赖性抗原。荧光标记的载体有助于通过流式细胞术研究B细胞亚群。
TNP hapten and Fluorescein reporter groups are conjugated to AminoEthylCarboxyMethyl-FICOLL (AECM-FICOLL), a high molecular weight polysaccharide. Hapten-FICOLL conjugates are useful as T-independent antigens for studying B lymphocyte activation. Fluorescently labeled carriers are helpful in studying B cell subsets by flow cytometry.
Properties:
Chemical Name: 2,4,6-Trinitrophenyl -AminoEthylCarboxyMethyl-FICOLL-Fluorescein;
Appearance: orange powder;
Absorption Maximum (Lambda Max): 348;
Extinction Coefficient at Lambda max: 15400;
Product Usage:
This product can be dissolved in water or phosphate buffered saline (PBS) pH 7.2, 0.15M, at a concentration of 1 mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. Store solution in aliquots at -20°C. Protect from light. Conjugation of TNP is given as a molar ratio of TNP to Ficoll assuming the molecular weight of Ficoll is 400,000. It is determined at 348 nm using 1.54 x 10E+03 as the extinction coefficient. Conjugation of Fluorescein is given as a molar ratio of Fluorescein to Ficoll assuming the molecular weight of Ficoll is 400,000. It is determined at 495 nm using 8.3 x 10E+04 as the extinction coefficient.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: Room Temperature
