详细描述：
NP（4-羟基-3-硝基苯基乙酸）半抗原与氨基乙基羧基甲基-聚蔗糖（AECM-FICOLL，一种高分子量多糖）偶联。 Hapten-FICOLL偶联物可用作研究B淋巴细胞活化的T非依赖性抗原。
NP (4-Hydroxy-3-nitrophenylacetic) hapten is conjugated to AminoEthylCarboxyMethyl-FICOLL (AECM-FICOLL), a high molecular weight polysaccharide. Hapten-FICOLL conjugates are useful as T-independent antigens for studying B lymphocyte activation.
Properties:
Chemical Name: 4-Hydroxy-3-nitrophenylacetic AminoEthylCarboxyMethyl-FICOLL;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 4230;
Product Usage:
Conjugation is determined at pH 8.5 by measuring NP absorbance at 430nm, using the extinction coefficient of NP= 4.23 x 10E+03. The conjugation ratio is given as a molar ratio of NP to AECM-FICOLL assuming the molecular weight of AECM-FICOLL is 400,000. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: Room Temperature
