详细描述：
荧光素与NP-氨基乙基羧基甲基-FICOLL（高分子量多糖）偶联。 Hapten-FICOLL偶联物可用作研究B淋巴细胞活化的T非依赖性抗原。
Fluorescein is conjugated to NP-AminoEthylCarboxyMethyl-FICOLL, a high molecular weight polysaccharide. Hapten-FICOLL conjugates are useful as T-independent antigens for studying B lymphocyte activation.
Properties:
Chemical Name: 4-Hydroxy-3-nitrophenylacetic -AminoEthylCarboxyMethyl-FICOLL-Fluorescein;
Appearance: orange powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 4230;
Product Usage:
This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. Store solution in aliquots at -20°C. Protect from light. Conjugation is determined at pH 8.5 by measuring NP absorbance at 430 nm, using 4.23 x 10E+03 as the extinction coefficient for NP, and is given as a molar ratio of NP to Ficoll assuming the molecular weight of Ficoll is 400,000. Conjugation for Fluorescein, is determined by measuring Fluorescein absorbance at 495 nm, using 8.3 x 10E+04 as the extinction coefficient for Fluorescein, and is given as a molar ratio of Fluorescein to Ficoll assuming the molecular weight of Ficoll is 400,000.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: Room Temperature
