详细描述:
5,6羧基荧光素通过酰胺键与卵清蛋白结合。
5,6 Carboxyfluorescein is conjugated to Ovalbumin protein through amide bonds.
Properties:
Chemical Name: Fluorescein-Ovalbumin;
Appearance: Orange powder;
Absorption Maximum (Lambda Max): 495;
Extinction Coefficient at Lambda max: 78000;
Product Usage:
Conjugation is determined by measuring fluorescein absorbance at 495nm, using the extinction coefficient of fluorescein, 8.3 x 10E+04. The conjugation ratio is given as a molar ratio fluorescein to ovalbumin assuming the molecular weight of ovalbumin is 45,000. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C