详细描述：
4-羟基-3-碘-5-硝基苯基乙酸活性酯可用于与蛋白质偶联，以形成用于产生抗体的免疫原性试剂。
4-Hydroxy-3-iodo-5-nitrophenylacetic active ester is useful for conjugation to proteins, in order to form immunogenic agents used to raise antibodies.
Properties:
Chemical Name: 4-Hydroxy-3-iodo-5-nitrophenylacetic acid Succinimide Ester;
Formula: C12H9IN2O7;
Molecular Weight: 420.12;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 5000;
Product Usage:
For conjugation to protein or DNA, dissolve in DMSO. NIP OSu Conjugation to a protein: Add NIP OSu to protein pre-dissolved in HCO3- buffer (pH ca 8.0) (Protein Concentration: Approx. 10 mg/mL). Stir 1 day at room temperature. Spin down insoluble material. Decant soluble fraction and dialyze until no color escapes (2-3 days). Lyophilize. Determine substitution ratio @430 nm, pH 8.5 Take a spectral scan of the solution using a spectrophotometer. Absorbance should be less than 1.0. Use the absorbance value at 430 nm to determine the loading of NIP per unit of OSu.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: -15 to -30 °C
