详细描述:
4-羟基-3-碘-5-硝基苯基乙酰基半抗原通过酰胺键通过赖氨酸与BSA蛋白偶联。
4-Hydroxy-3-iodo-5-nitrophenylacetyl hapten is conjugated to BSA protein through lysine by amide bonds.
Properties:
Chemical Name: 4-Hydroxy-3-iodo-5-nitrophenylacetyl bovine serum albumin;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 5000;
Product Usage:
Conjugation is determined at pH 8.5 by measuring NIP absorbance at 430nm, using the extinction coefficient of NIP= 5.0 x 10E+03. The conjugation ratio is given as a molar ratio of NIP to BSA assuming the molecular weight of BSA is 66,430. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C