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NIP-Fluorescein-BSA (牛血清白蛋白)

NIP-Fluorescein-BSA (Bovine Serum Albumin)

产品概述 技术指标 订购信息 服务支持
详细描述：
荧光素通过酰胺键与4-羟基-3-碘-5-硝基苯基乙酰基BSA赖氨酸偶联。 Fluorescein is conjugated to 4-Hydroxy-3-iodo-5-nitrophenylacetyl BSA lysine through amide bonds.
Properties: Chemical Name: 4-Hydroxy-3-iodo-5-nitrophenylacetyl Bovine Serum Albumin Fluorescein; Appearance: yellow powder; Absorption Maximum (Lambda Max): 430; Extinction Coefficient at Lambda max: 5000; Product Usage: Conjugation is determined at pH 8.5 by measuring NIP absorbance at 430nm, using the extinction coefficient of NIP= 5.0 x 10E+03. The conjugation ratio is given as a molar ratio of NIP to BSA assuming the molecular weight of BSA is 66,430. Conjugation of Fluorescein is determined at 495 nm using 8.3 x 10E+04 as the extinction coefficient. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water; Storage and Handling: Shipping Conditions: Ambient; Storage Conditions: +2 to +8 °C
货号 名称 单位 购买
N-5040F-10 NIP-Fluorescein-BSA (牛血清白蛋白) 10 mg 商城订购

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