详细描述:
4-羟基-3-硝基苯基乙酰基半抗原通过酰胺键与HEL(母鸡溶菌酶)赖氨酸偶联。
4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to HEL (Hen Egg Lysozyme) lysine through amide bonds.
Properties:
Chemical Name: 4-Hydroxy-3-nitrophenylacetyl Hen Egg Lysozyme;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 4230;
Product Usage:
This product can be dissolved in 0.1M NaHCO3 buffer, pH 8.3-8.5 or DMSO at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. Store solution in aliquots at -20°C. Protect from light. Conjugation is determined at pH 8.5 by measuring NP absorbance at 430 nm, using 4.23 x 10E+03 as the extinction coefficient for NP, and is given as a molar ratio of NP to HEL assuming the molecular weight of HEL is 14,300.
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C