详细描述：
4-羟基-3-硝基苯基乙酰基半抗原通过酰胺键与KLH（钥孔血蓝蛋白）赖氨酸偶联。
4-Hydroxy-3-nitrophenylacetyl hapten is conjugated to KLH (Keyhole Limpet Hemocyanin) lysine through amide bonds.
Properties:
Chemical Name: 4-Hydroxy-3-nitrophenylacetyl-Keyhole Limpet Hemocyanin;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 430;
Extinction Coefficient at Lambda max: 4230;
Product Usage:
Conjugation is determined at pH 8.5 by measuring NP absorbance at 430nm, using the extinction coefficient of NP= 4.23 x 10E+03. The conjugation ratio is given as a molar ratio of NP to KLH assuming the molecular weight of KLH is 100,000. Store solution in aliquots at +5°C. KLH will come out of solution if frozen. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when dissolved or stored in solution, therefore it is recommended to sonicate the solution for 30 minutes before use. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C
