详细描述:
2,4-二硝基苯基半抗原通过赖氨酸与卵清蛋白结合。
2,4-Dinitrophenyl hapten is conjugated to Ovalbumin protein through lysine.
Properties:
Chemical Name: 2,4-Dinitrophenyl-Ovalbumin;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 360;
Extinction Coefficient at Lambda max: 17400;
Product Usage:
Conjugation for DNP is determined by measuring the absorbance at 360 nm and using the extinction coefficient of DNP =1.74 x 10E+04. The conjugation ratio is given as the molar ratio of DNP to Ovalbumin assuming the molecular weight of Ovalbumin is 45,000. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in water or phosphate buffered saline (PBS) pH 7.2 at a concentration of 1 mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C