详细描述:
磷酰胆碱半抗原通过偶氮键与对羟基苯乙酸偶联,该部分然后通过水溶性碳二亚胺偶联偶联至BSA载体。
Phosphorylcholine hapten is coupled to p-hydroxyphenylacetic acid through azo linkage; this moiety then coupled to BSA carrier via water soluble carbodimide conjugation.
Properties:
Chemical Name: Phosphorylcholine p-hydroxy phenylacetic acid Bovine Serum Albumin;
Formula: C11H17N3O4P-HPA-BSA;
Molecular Weight: 286.25;
Appearance: yellow powder;
Absorption Maximum (Lambda Max): 475;
Extinction Coefficient at Lambda max: 12600;
Product Usage:
Conjugation is determined at pH 12 by measuring PC absorbance at 475nm, using the extinction coefficient of PC= 12.6 x 10E+03. The conjugation ratio is given as a molar ratio of PC HPA to BSA assuming the molecular weight of BSA is 66,430. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C