详细描述:
荧光素与PC-BSA偶联。该产品与PC-BSA同源,在细胞流式细胞术中更为有用。
Fluorescein is conjugated to PC-BSA. This product is homologous to PC-BSA and is most useful in cell cytometry.
Properties:
Chemical Name: PC-BSA-Fluorescein;
Appearance: orange powder;
Absorption Maximum (Lambda Max): 475;
Extinction Coefficient at Lambda max: 12600;
Product Usage:
Conjugation is determined at pH 13 by measuring PC absorbance at 475nm, using the extinction coefficient of PC= 12.6 x 10E+03. The conjugation ratio is given as a molar ratio of PC to BSA assuming the molecular weight of BSA is 66,430. Conjugation for Fluorescein, is determined by measuring Fluorescein absorbance at 495 nm, using 8.3 x 10E+04 as the extinction coefficient for Fluorescein, and is given as a molar ratio of Fluorescein to BSA assuming the molecular weight of BSA is 66,430. Store solution in aliquots at -20°C. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C