详细描述：
磷酸胆碱半抗原通过使用对重氮苯基磷酸胆碱与KLH蛋白偶联。
Phosphorylcholine hapten is conjugated to KLH protein by use of p-diazonium phenylphosphorylcholine.
Properties:
Chemical Name: Phosphorylcholine Keyhole Limpet Hemocyanin;
Formula: C11H17N3O4P-KLH;
Molecular Weight: 286.25;
Appearance: orange powder;
Absorption Maximum (Lambda Max): 475;
Extinction Coefficient at Lambda max: 12600;
Product Usage:
Conjugation is determined at pH 12 by measuring PC absorbance at 475nm, using the extinction coefficient of PC= 12.6 x 10E+03. The conjugation ratio is given as a molar ratio of PC to KLH assuming the molecular weight of KLH is 100,000. Store solution in aliquots at +5°C. KLH may come out of solution if frozen. Protect from light. This product can be dissolved in PBS buffer, water or other neutral buffers at a concentration of 1mg/mL. Product may precipitate when stored in solution, therefore it is recommended to centrifuge the solutions briefly before use and use only the supernatant. When using PBS please refer to the formulation below. PBS Formulation: 8 g NaCl 0.2 g KCl 0.2 g potassium di-hydrogen phosphate (KH2PO4) 1.15 g di-sodium hydrogen phosphate (Na2HPO4) Add to 1 L of water;
Storage and Handling:
Shipping Conditions: Ambient;
Storage Conditions: +2 to +8 °C
