KASP by Design (KBD) consists of three KASP primers that are specific to the SNP or InDel of interest. Assay design (primer selection) is carried out using our proprietary Kraken™ software system.
Please note that using in-silico validated primers cannot guarantee a working assay. The success rate for custom KASP assay design depends on a number of factors including DNA quality, genome complexity, homology and genome size. Any custom KASP assay can be provided with our additional assay validation service to ensure a working assay.
Aliquots of KBD Assay mix are provided in 2D barcoded tubes in sufficient quantities to enable:
2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
5,000 reactions at 5 µL total reaction volumes (384-well PCR plates).
Standard delivery times for KBD Assay mix is 2-3 weeks. Please note that you will require KASP Master mix in order to run your genotyping reactions. Please refer to the KASP Master mix – PCR instrument guide to select the correct KASP Master mix for your instrument.
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KASP Primer Mix存放于单个带有二维码的试剂管中,便于管理与追踪:
用96孔PCR板,10 uL体系,可以检测2500次反应;
用384孔PCR板,5 uL体系,可以检测5000次反应。
Aliquots of KBD Assay mix are provided in 2D barcoded tubes in sufficient quantities to enable:
2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
5,000 reactions at 5 µL total reaction volumes (384-well PCR plates).
Standard delivery times for KBD Assay mix is 2-3 weeks. Please note that you will require KASP Master mix in order to run your genotyping reactions. Please refer to the KASP Master mix – PCR instrument guide to select the correct KASP Master mix for your instrument.