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KBD引物混合物

KASP by Design Primer Mix

产品概述 技术指标 订购信息 服务支持
详细描述:
KBD引物mix包含3条针对SNP或InDel特异性的KASP引物:两条等位基因特异性的正向引物和一条通用的反向引物,引物设计与筛选在LGC的Kraken软件系统上完成。

注意:使用在硅片验证的引物不能保证实验100%有效。KASP实验成功的几率依赖于一系列因素,包括DNA质量,基因组复杂性,同源性以及基因组大小。使用KOB引物可以保证实验100%有效。

订购需填写订购表格。
KASP Primer Mix存放于单个带有二维码的试剂管中,便于管理与追踪:
用96孔PCR板,10 uL体系,可以检测2500次反应;
用384孔PCR板,5 uL体系,可以检测5000次反应。

KBD引物的标准设计周期是2-3周,注意一个完整的基因分型实验除了KASP引物mix之外还需要KASP Master Mix,请根据您PCR仪器的型号选择合适的Master Mix。

KASP by Design (KBD) consists of three KASP primers that are specific to the SNP or InDel of interest. Assay design (primer selection) is carried out using our proprietary Kraken™ software system.

Please note that using in-silico validated primers cannot guarantee a working assay. The success rate for custom KASP assay design depends on a number of factors including DNA quality, genome complexity, homology and genome size. Any custom KASP assay can be provided with our additional assay validation service to ensure a working assay.

Aliquots of KBD Assay mix are provided in 2D barcoded tubes in sufficient quantities to enable:
2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
5,000 reactions at 5 µL total reaction volumes (384-well PCR plates).

Standard delivery times for KBD Assay mix is 2-3 weeks. Please note that you will require KASP Master mix in order to run your genotyping reactions. Please refer to the KASP Master mix – PCR instrument guide to select the correct KASP Master mix for your instrument.
订购需填写订购表格。
KASP Primer Mix存放于单个带有二维码的试剂管中,便于管理与追踪:
用96孔PCR板,10 uL体系,可以检测2500次反应;
用384孔PCR板,5 uL体系,可以检测5000次反应。

KBD引物的标准货期是2-3周,注意一个完整的基因分型实验除了KASP引物mix之外还需要KASP Master Mix,请根据您PCR仪器的型号选择合适的Master Mix。

Aliquots of KBD Assay mix are provided in 2D barcoded tubes in sufficient quantities to enable:
2,500 reactions at 10 µL total reaction volumes (96-well PCR plates)
5,000 reactions at 5 µL total reaction volumes (384-well PCR plates).

Standard delivery times for KBD Assay mix is 2-3 weeks. Please note that you will require KASP Master mix in order to run your genotyping reactions. Please refer to the KASP Master mix – PCR instrument guide to select the correct KASP Master mix for your instrument.
货号 名称 单位 购买
KBS-2100-100-OLI KBD引物混合物 2,500 x 10ul Reactions(国内合成业务) 商城订购

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