The validation kit is supplied to ensure that the plate-reader, PCR thermal cycler and process flow are functioning correctly for use with KASP™ chemistry. In general, any qPCR instrument or FRET-capable plate reader should be compatible with KASP. The different instruments have differing requirements for ROX™, the passive reference dye present in KASP Master mix, and you may need to try more than one version to determine the version that is optimal for your instrument. KASP Master mix is available in Low, Standard and High ROX formulations. Please note: these formulations only differ in the level of ROX that they contain and are otherwise identical.
