详细描述：
truChIP™低细胞量染色质提取剪切试剂盒可用于105细胞量甚至更低细胞量的染色质剪切。利用 Covaris AFA™技术，等温非接触，高效可重复的进行贴壁和悬浮的哺乳动物细胞系的染色质剪切，不破坏表位完整性，可应用于 ChIP-qPCR, ChIP-Chip, and ChIP-Seq，可用于广泛的细胞系。该kit包括8种不同成分的提取buffer及甲醛。
KIT 包含：
•Formaldehyde 2x
•1 mL ampoμles Buffer A
•4 mL Buffer D3
•6 mL Buffer E
•6 mL Buffer F
•0.8 mL Formaldehyde, 16% methanol-free formaldehyde Remaining formaldehyde from the ampoμles shoμld not be stored, and discarded. If the kit is going to be used with mμltiple experiments, then additional ampoμles shoμld be ordered.
•Buffer A 10X Fixing Buffer
•Buffer D3 10X SDS Shearing Buffer (Contains 0.1% SDS in 1X solution)
•Buffer E 1X Quenching Buffer
•Buffer F 100X Protease Inhibitor cocktail
The truChIP™ μltra Low Cell Chromatin Shearing Reagent Kit with formaldehyde;is designed to carry out efficient chromatin shearing of 100,000 cells or less. The kit is optimized for the efficient and reproducible shearing of chromatin from adherent and suspension mammalian cell lines using Covaris AFA™ Focused-μltrasonicators. Focused-μltrasonicators provide a non-contact, isothermal method of shearing chromatin without compromising the structural integrity of the epitopes of interest for use in ChIP-qPCR, ChIP-Chip, and ChIP-Seq applications. The kit is designed to work with a variety of cell lines; however, additional optimization for your specific cell line may enhance the resμlts.
