详细描述：
GetClone™ PCR克隆载体 (GetClone™ PCR Cloning vector, CV1000)为高效能之正向筛选系统克隆载体，适合于克隆由高保真聚合酶所产生之平末端PCR产物或平端DNA片段。 此克隆载体含有一致死基因，于克隆位置接合一外来DNA片段，就会破坏致死基因的表现，则细菌寄主能正常生长，相反的，当载体自行接合 (self-ligation)则可正常表现致死基因并杀死细菌本身。 因此只有含插入DNA片段之载体的细菌能够生长为菌落，也因此不需IPTG and X-Gal 之蓝/白筛选便可达到筛选目的。The GetClone™ PCR Cloning Vector is a positive selection system for the high efficiency cloning of blunt end DNA or amplicons. This cloning vector contains a lethal gene which can be disrupted by ligation with a blunt end DNA insert at the cloning site. Only colonies with inserted vectors are able to propagate, eliminating the additional needs of IPTG and X-Gal for blue/ white screening.
