详细描述:
常用的 DNA 底物,双链 DNA,从 lambda 噬菌体(在 dam- 大肠杆菌宿主菌中培养)中分离得到。该 DNA 对有 dam 甲基化活性的内切酶敏感。该 DNA 长度为 48,502 bp。Duplex DNA is isolated from bacteriophage lambda (cI857ind 1 Sam 7).The bacteriophage is grown in an E. coli host which is deficient in adenine methylase (dam-).This DNA gives complete digests with restriction endonucleases that are sensitive to dam methylation. Lambda DNA (N6-methyladenine-free) is 48,502 base pairs in length.