详细描述：
此抗体套装包含有Phospho-IRS-1 (Ser307) Antibody #2381T、Phospho-IRS-1 (Ser1101) Antibody #2385T、Phospho-IRS-1 (Ser636/639) Antibody #2388T、Phospho-IRS-1 (Ser302) (34C7) Rabbit mAb #2491T、Phospho-IRS-1 (Ser612) (C15H5) Rabbit mAb #3203T、IRS-1 (D23G12) Rabbit mAb #3407T、Phospho-IRS-1 (Ser318) (D51C3) Rabbit mAb #5610T和Anti-rabbit IgG, HRP-linked Antibody #7074P2。IRS-1 Inhibition Antibody Sampler试剂盒提供了经济的方式通过多种IRS-1丝氨酸残基的磷酸化状态检测胰岛素信号通路的负反馈过程。该试剂盒提供了足以进行4次western blot分析的一抗。每个活化状态的抗体可以识别其各自靶点的磷酸化形式。所有的靶点残基都是基于小鼠的IRS-1序列的，除了Ser636/339和Ser1101，是基于人IRS-1的序列。IRS-1 (D23G12) Rabbit mAb可以识别总的IRS-1蛋白而不管其磷酸化状态。合成对应IRS-1的mouse Ser307 (human Ser312), human Ser636/639 (mouse Ser632/635), 和 human Ser1101 (mouse Ser1097)的磷酸多肽免疫动物获得多抗。多抗由蛋白A和肽段亲和层析技术纯化得到。合成对应IRS-1的mouse Ser302 (human Ser307), mouse Ser318 (human Ser323), 和 mouse Ser612 (human 616)的磷酸多肽免疫动物获得单抗。IRS-1 (D23G12) rabbit monoclonal兔单抗通过合成与human IRS-1 的Ser270(mouse 1097)邻近序列一致的多肽免疫动物获得。The IRS-1 Inhibition Antibody Sampler Kit provides an economical means to evaluate insulin signaling negative feedback loops via phosphorylation of various IRS-1 serine residues. The kit includes enough antibody to perform four western blot experiments with each primary antibody.Each activation state antibody recognizes the phosphorylated form of its target. All target residues are based on the sequence for mouse IRS-1, except Ser636/339 and Ser1101, which are based on the sequence for human IRS-1. IRS-1 (D23G12) Rabbit mAb recognizes total IRS-1 protein independent of its phosphorylation state.Polyclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding mouse Ser307 (human Ser312), human Ser636/639 (mouse Ser632/635), and human Ser1101 (mouse Ser1097) of IRS-1. Polyclonal antibodies are purified by protein A and peptide affinity chromatography. Monoclonal antibodies are produced by immunizing animals with synthetic phosphopeptides corresponding to residues surrounding mouse Ser302 (human Ser307), mouse Ser318 (human Ser323), and mouse Ser612 (human 616) of IRS-1. IRS-1 (D23G12) rabbit monoclonal antibody is produced by immunizing animals with a synthetic peptide corresponding to the sequence surrounding Ser270 of human IRS-1 (mouse 1097).
