详细描述：
此抗体套装包含Shh (C9C5) Rabbit mAb #2207、PTCH1 (C53A3) Rabbit mAb #2468、PTCH2 (G1191) Antibody #2470、SUFU (C54G2) Rabbit mAb #2520、GLI1 (C68H3) Rabbit mAb #3538各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。此抗体样品试剂盒提供经济节省方式来检测Hedgehog信号通路蛋白。.这种试剂盒包含足够做四次小的western blot实验所需的一抗和二抗。Shh (C9C5)兔源单克隆蛋白识别内源性的总Shh 蛋白。此抗体不与转染的IHH和DHH交叉反应。PTCH1 (C53A3)兔源单克隆抗体识别转染水平的PTCH1蛋白。此抗体也通过它免疫沉淀后的Western blot 分析检测内源性PTCH1的水平。PTCH2(G1191)抗体识别转染水平的PTCH2蛋白。它不能识别转染的PTCH1蛋白的人源PTCH1蛋白。SUFU (C54G2)兔源单克隆抗体识别内源性的总SUFU蛋白。GLI1 (V812)抗体识别内源性的总GLI1 蛋白。This sampler kit provides an economical means of evaluating key members of the Hedgehog signaling pathway. The kit contains enough primary and secondary antibody to perform four western miniblot experiments.Shh (C9C5) Rabbit mAb detects endogenous levels of total Shh protein. This antibody does not cross-react with transfected IHH and DHH. PTCH1 (C53A3) Rabbit mAb detects transfected levels of PTCH1. This antibody can also detect endogenous levels of PTCH1 through immunoprecipitation followed by Western blot analysis. PTCH2 (G1191) Antibody detects transfected levels of PTCH2 protein. It does not recognize transfected levels of human PTCH1 protein. SUFU (C54G2) Rabbit mAb detects endogenous levels of total SUFU protein. GLI1 (V812) Antibody detects endogenous levels of total GLI1 protein.
