Actin Nucleation and Polymerization Antibody Sampler Kit
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此抗体套装包含Phospho-Rac1/cdc42 (Ser71) Antibody #2461、ARP2 Antibody #3128、Profilin-1 (C56B8) Rabbit mAb #3246、WAVE-2 (D2C8) XP® Rabbit mAb #3659、Rac1/Cdc42 Antibody #4651、ARP3 Antibody #4738、N-WASP (30D10) Rabbit mAb #4848各20µl,以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Actin Nucleation and Polymerization Antibody Sampler 试剂盒提供了一种经济的方式对肌动蛋白丝成核和多聚化的存在以及状态进行检测。试剂盒含有足以进行4次western blot实验的一抗。ARP2抗体可以识别内源性总的ARP2蛋白。ARP3抗体可以识别内源性总的ARP3蛋白。抗体与内源性ARP2蛋白没有交叉反应。Profilin-1 (C56B8)兔mAb可以识别内源性总的profilin-1蛋白。抗体可能和profilin-2蛋白油交叉反应。Phospho-Rac1/cdc42 (Ser71)抗体仅可以识别71位丝氨酸磷酸化的内源性Rac1/cdc42。抗体可能也能检测phospho-RhoA (Ser73). Rac1/Cdc42抗体可以识别内源性总的rac1和cdc42蛋白。基于序列相似性,该抗体也可能能与rac2和rac3反应;抗体与RhoA, RhoB, 或RhoC无交叉反应。N-WASP (30D10)兔mAb可以识别内源性总的N-WASP蛋白。该抗体与造血干细胞特异蛋白,WASP,无交叉反应。WAVE-2 (D2C8) XP®兔mAb可以识别内源性总的WAVE-2蛋白。The Actin Nucleation and Polymerization Antibody Sampler Kit provides an economical means to evaluate the presence and status of actin nucleation and polymerization. The kit contains enough primary antibody to perform four western blots per primary.ARP2 Antibody recognizes endogenous levels of total ARP2 protein. ARP3 Antibody recognizes endogenous levels of total ARP3 protein. This antibody does not cross-react with endogenous ARP2. Profilin-1 (C56B8) Rabbit mAb recognizes endogenous levels of total profilin-1 protein. This antibody may cross-react with profilin-2. Phospho-Rac1/cdc42 (Ser71) Antibody recognizes endogenous levels of Rac1/cdc42 only when phosphorylated at serine 71. This antibody may also detect phospho-RhoA (Ser73). Rac1/Cdc42 Antibody recognizes endogenous levels of total rac1 and cdc42 proteins. Based on sequence similarities, this antibody may also detect rac2 and rac3; this antibody does not cross-react with RhoA, RhoB, or RhoC. N-WASP (30D10) Rabbit mAb recognizes endogenous levels of total N-WASP protein. This antibody does not cross-react wth the hematopietic protein, WASP. WAVE-2 (D2C8) XP® Rabbit mAb recognizes endogenous levels of total WAVE-2 protein.