详细描述:
此抗体套装包含Atg12 Antibody (Human Specific) #2010、LC3B (D11) XP® Rabbit mAb #3868、LC3A (D50G8) XP® Rabbit mAb #4599各20µl,以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl。Autophagosome Marker Antibody Sampler 试剂盒提供了一种经济有效的方法,能够检测细胞中积累的自噬体。这种试剂盒含有足够的一抗和二抗,每个一抗可以进行四次western blots。Autophagosome Marker Antibody Sampler 试剂盒中的每个抗体可以检测到内源性的目标总蛋白。Atg12抗体(人源特异性)能够识别游离的Atg12或Atg12-Atg15复合物。LC3A (D50G8) XP® Rabbit mAb 兔单抗和 LC3B (D11) XP® Rabbit mAb 兔单抗能够与其它LC3异构体发生交叉反应,并且对于II型LC3的反应要更强。The Autophagosome Marker Antibody Sampler Kit provides an economical means to investigate the accumulation of autophagosomes within the cell. The kit contains enough primary and secondary antibodies to perform four western blots per primary antibody.Each antibody in the Autophagosome Marker Antibody Sampler Kit detects endogenous levels of total respective protein. Atg12 Antibody (Human Specific) recognizes free Atg12 and the Atg12-Atg5 conjugate. LC3A (D50G8) XP® Rabbit mAb and LC3B (D11) XP® Rabbit mAb may cross-react with other LC3 isoforms and have stronger reactivity with the type II form.