详细描述：
此套装包含pCMV-Tag4A-mBad/GrpE 20µg，Phospho-Bad (Ser136) (D25H8) Rabbit mAb #4366、Phospho-Bad (Ser112) (40A9) Rabbit mAb #5284、Bad (D24A9) Rabbit mAb #9239、Phospho-Bad (Ser155) Antibody #9297各20µl，以及含Anti-rabbit IgG, HRP-linked Antibody #7074 100µl.Phospho-Bad Antibody Sampler Kit 提供了一种经济的方法研究Bad蛋白在凋亡中的作用。试剂盒中所包含的一抗和二抗，足够用于四次Western blots免疫印记。Bad (D24A9) Rabbit mAb 兔单抗能够检测内源性的Bad总蛋白。Phospho-Bad (Ser112) (40A9) Rabbit mAb 兔单抗只能检测内源性的Ser112位点磷酸化的Bad。Ser112的系统命名法是基于小鼠蛋白序列。在人和大鼠中，其同工酶对应的分别是Ser75和Ser113位点。Phospho-Bad (Ser136) (185D10) Rabbit mAb 兔单抗能够检测免疫共沉淀的或者转染水平的Ser136 磷酸化的 Bad 蛋白。Ser136系统命名法是基于小鼠的蛋白序列，其同工蛋白在人和大鼠中的位点分别是Ser99和Ser137。Phospho-Bad (Ser155) Antibody 能够检测转染的Ser155位点磷酸化的Bad蛋白。Ser155 的系统命名法是基于小鼠的序列，其在人和大鼠中同工酶的位点分别是Ser118 和 Ser156 位点。The Phospho-Bad Antibody Sampler Kit provides an economical means to investigate the role of Bad protein in apoptosis. The kit contains primary and secondary antibodies to perform four Western blots with each antibody.Bad (D24A9) Rabbit mAb detects endogenous levels of total Bad protein. Phospho-Bad (Ser112) (40A9) Rabbit mAb detects endogenous levels of Bad only when phosphorylated at Ser112. The Ser112 nomenclature is based upon the mouse sequence. The analogous phosphorylation sites are Ser75 in human and Ser113 in rat. Phospho-Bad (Ser136) (185D10) Rabbit mAb detects immunoprecipitated or transfected levels of Bad protein only when phosphorylated at Ser136. The Ser136 nomenclature is based upon the mouse sequence. The analogous phosphorylation sites are Ser99 in human and Ser137 in rat. Phospho-Bad (Ser155) Antibody detects transfected levels of Bad only when phosphorylated at Ser155. The Ser155 nomenclature is based upon the mouse sequence. The analogous phosphorylation sites are Ser118 in human and Ser156 in rat.
