详细描述：
BHQplus®探针是一种用于qPCR实验的新型探针，采用了双重稳定技术进行修饰，并用Biosearch的Black Hole Quencher®染料标记在探针末端进行淬灭。BHQplus探针与其他传统qPCR探针的区别在于其允许设计较短的寡核苷酸（通常长度为15-25个碱基），可用于检测更复杂困难的靶序列，特异性更强，是对单核苷酸多态性SNP进行基因分型的理想选择。
BHQplus探针提供两种形式：1）若需要BHQplus探针和引物分开单独包装，选择“BHQplus探针”；2）如果希望探针和引物混合在单管中，则选择“ValuMix Assay for SNP geneotyping”。
用于SNP基因分型的ValuMix试剂包含两个定制的BHQplus®探针和两个引物混合在一个单管中。这个混合mix形式能够更小化移液误差并简化实验流程，可直接用于SNP基因分型。
BHQplus探针特点：
小但强大：双稳态允许设计较短的双标记探针
高保真度：较短的序列特异性更强，能够确保错配杂交率更低
经过验证的性能：黑洞淬灭剂染料确保在目标杂交之前完全熄灭荧光
探针设计简便：通过Biosearch免费的在线工具RealTimeDesign™软件就可以设计得到BHQplus探针和引物
底线：基于探针的SNP基因分型的经济替代方法
请注意，长于25个碱基的序列可以被设计为传统探针，在BHQplus形式中可能不能正常工作。如果这样的序列已经具有70℃的预测熔融温度，则可以考虑订购BHQ®探针。相反，短于15个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且应该首先分析与BHQplus化学的相容性。有关探头设计的任何问题，请联系基因有限公司技术人员。
The BHQplus® probe is a novel compact probe for qPCR, modified with a duplex stabilizing technology and terminated with Biosearch’s Black Hole Quencher® dye. What differentiates the BHQplus probe from other traditional probes is that it permits the design of shorter oligonucleotides, typically 15 - 25 bases in length, for detecting more difficult target sequences. The enhanced specificity of BHQplus probes make them perfect for genotyping single nucleotide polymorphisms.
BHQplus probes are offered in two convenient deliveries. For BHQplus probes and primers delivered in individual tubes select just “BHQplus probes”, however if you would like the probes and primers blended into a single tube select “ValuMix Assay for SNP genotyping”.
ValuMix™ Assays for SNP genotyping comprises two custom BHQplus® probes and two primers combined within a single tube. This formulation minimizes pipetting error and simplifies your protocol by providing mixed oligonucleotides ready for SNP genotyping.
Please refer to the Product Listings tab to view all dye-quencher combinations and delivery quantities available.
BHQplus probes feature:
1. Small but powerful: duplex stabilizers permit the design of shorter dual-labeled probes
2. High fidelity: shorter sequences offer an increased barrier against hybridizing to a mismatch
3. Proven performance: the Black Hole Quencher dye extinguishes fluorescence until target hybridization
4. A mouse-click away: design your BHQplus probe through Biosearch’s free, web-based RealTimeDesign™ software
5. Bottom line: An economical alternative for probe-based SNP Genotyping
Please be aware that sequences longer than 25 bases may have been designed as a traditional probe and are unlikely to work well in BHQplus form. Instead, consider ordering a BHQ® probe instead if such a sequence already has a predicted melting temperature of 70 °C. Conversely, sequences shorter than 15 bases may have been designed as a Minor Groove Binder (MGB™) probe and should first be analyzed for compatibility with the BHQplus chemistry. Please contact info@genecompany.com with any questions regarding probe design.
NOTES:
Building Probes with Wobble/Degenerate Bases (What are Wobbles?)
To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc.
Biosearch Technologies offers RealTimeDesign™ software, a FREE assay design program hosted on the web. Start designing your BHQplus probes and primer pairs today!
