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双标记探针, 5' CAL Fluor® Red 635/3' BHQ-2

Dual-labeled Probe, 5' CAL Fluor® Red 635/3' BHQ-2

产品概述 技术指标 订购信息 服务支持
详细描述：
双标记的BHQ探针是一种传统的线性FRET探针，其将荧光基团和淬灭基团共价连接到通常长度为20至30个碱基的寡核苷酸的5'和3'末端。荧光通过Taq聚合酶的5'外切核酸酶活性释放，Taq聚合酶在探针与其互补序列杂交时切割荧光染料。双标记BHQ探针是检测特异性靶序列的存在和定量的理想选择。 BHQ探针提供两种形式： 1）探针和引物在分开在单独管中形式； 2）用于基因表达和qPCR的ValuMix形式，即在单管中包含一个探针和两个引物的混合物。 ValumixPrimer的数量可以根据指定的探针量进行灵活地调整，如0.5 nmol，5 nmol或20 nmol。探针与引物的比率可以根据您的喜好在1：1至1：4.5的范围内。ValuMix是一个经济的选择，LGC Biosearch以经济实惠的价格提供0.5 nmol超小包装FAM-BHQ探针引物混合物。有关完整的选择范围，请参阅官网产品列表选项卡。 BHQ探针设计注意事项： 双标记探针作为一种末端标记探针，当序列长于30个碱基时会影响荧光淬灭效率。对于这种情况，请考虑BHQnova双猝灭探针（https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhqnova-probes）或将猝灭剂放在探针中间位置设计具有内部猝灭的探针（该形式当前仅提供FAM标记）。相反，短于20个碱基的序列可以被设计为小沟结合剂（MGB）探针，并且不可能在70℃的适当温度下结合。请考虑订购BHQplus®探针，或者联系基因有限公司技术人员咨询有关探针设计的任何问题。 注意： 1. 设计具有内部猝灭的探针（当前仅提供FAM） 2. 添加由T（...）注释的内部修饰表示胸腺嘧啶碱基连接到内部修饰。请注意，在添加此类内部修饰时，将在序列中引入另外的T碱基。 3. 具有不确定/降解碱基的序列如何设计探针，请参考https://www.biosearchtech.com/support/faqs/custom-oligonucleotides-modifications/what-are-wobbles 4. 为了确保寡核苷酸能被有效识别，当在序列输入框中输入不确定/降解碱基时请使用IUPAC代码，而非括号，如：（A / G），（A / G / C / T）等。 Dual-Labeled BHQ Probes are traditional, linear, FRET probes incorporating a fluorophore and quencher covalently attached to the 5' and 3' ends of an oligo typically 20 to 30 bases in length. Fluorescence is released through the 5' exonuclease activity of Taq polymerase, which cleaves off the fluorescent dye upon the probe's hybridization to its complementary sequence. Dual-Labeled BHQ Probes are ideal for detecting the presence and quantifying the amount of specific target sequences. BHQ probes are offer delivery of the probe and primers in separate individual tubes. We also offer ValuMix for gene expression and qPCR which delivers one probe and two primers in a single tube. ValumixPrimer quantities can be flexibly adjusted around a specified probe amount of either 0.5 nmol, 5 nmol, or 20 nmol delivered. Probe to primer ratios can range anywhere from 1:1 to 1:4.5 according to your preference. ValuMix is an economical option providing 0.5 nmol of FAM-BHQ probe mixed with primers for only $79. See the product listing tab for full range of options. Design Concerns for BHQ probes Please be aware that sequences longer than 30 bases are unlikely to have efficient quenching as an end-labeled probe. For such situations please consider positioning the quencher internally instead. Conversely, sequences shorter than 20 bases may have been designed as a Minor Groove Binder (MGB™) probe and are unlikely to bind at the proper temperature of 70 °C. Please consider ordering such a sequence as a BHQplus® probe instead, or else contact info@genecompany.com with any questions regarding probe design. NOTE: Building Probes with an Internal Quencher (Currently available with FAM only) Adding an internal modification annotated by T(...) indicates that a Thymidine base is attached to the internal modification. Please be aware that an additional T base will be introduced into the sequence when adding such an internal modification. Building Probes with Wobble/Degenerate Bases (What are Wobbles?) To ensure the most efficient processing of your oligo, use IUPAC code when entering wobble/degenerate bases in the Sequence Entry field instead of using parentheses, e.g. (A/G), (A/G/C/T), etc. Best DEAL: ValuProbe™ BHQ Probe - 10 nanomoles of FAM-BHQ Probe typically ships in 2 business days Instrument CALIBRATION: FAM, CAL Fluor and Quasar calibration dyes are available here for online purchase Learn MORE: Applying dual-labeled probes in Multiplexing qPCR, Gene Expression, and SNP Genotyping assays The difference between FRET and static quenching Assay DESIGN: Try RealTimeDesign™ Software, a FREE, qPCR assay design program. Design your Dual-Labeled BHQ probes and primers online today!
https://www.biosearchtech.com/products/qpcr-and-snp-genotyping/bhq-probes#?tab=product-info
货号 名称 单位 购买
DLO-C635B2-2 双标记探针, 5' CAL Fluor® Red 635/3' BHQ-2 200 nmol 商城订购

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