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Stellaris原位杂交探针,Quasar® 570标记的人GAPDH探针

Stellaris® FISH Probes, Human GAPDH with Quasar 570® Dye

产品概述 技术指标 订购信息 服务支持
详细描述:
人GAPDH探针包括一组Quasar® 570标记的寡核苷酸以相同的比例混合在一起,终浓度是1 nmol,按照标准条件操作能够做大概80次杂交。该探针用于利用荧光原位杂交的方法检测人GAPDH的转录本。 探针设计依据:Product was designed against Human Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, a.k.a. G3PD; GAPD (NCBI gene ID: 2597), and the coding sequence of NM_002046.4 nts 175-1182. 该探针没有在同物种或其它物种的同源和直系同源序列上验证可能的交叉杂交。 Human GAPDH consists of a set of Quasar® 570-labeled oligos mixed at equal ratios and pooled into a final delivered amount of 1 nmol, which yields approximately 80 hybridizations under standard conditions. Designed to detect GAPDH transcripts in Human specimens using fluorescence in situ hybridization (FISH). Design Criteria: Product was designed against Human Glyceraldehyde-3-phosphate dehydrogenase, GAPDH, a.k.a. G3PD; GAPD (NCBI gene ID: 2597), and the coding sequence of NM_002046.4 nts 175-1182.The probe set has not been tested for potential cross-hybridization to RNA(s) of paralogous and orthologous gene(s) in the same or other species. Representative image of human GAPDH mRNAs detected with a Quasar 570 dye labeled probe set in A549 cells.
属性: 最大激发荧光(Lambda Max):548; 最大发射荧光:566; 产品用法: 溶解后的探针注意尽量避免反复冻融。当天或短期使用,探针溶液可以避光放在+2 ~ +8 °C储存一个月,超过1个月,推荐分装后将溶解后的探针放在-15至-30 °C避光保存。 Protocols:详细Protocol根据样本类型而异,请上以下网站下载:https://www.biosearchtech.com/stellarisprotocols; 储存及运输: 运输条件:常温/蓝冰/干冰; 储存条件:+2 ~ +8 °C; Properties: Product Usage: Absorption Maximum (Lambda Max): 647; Fluorescence Maximum: 670; Product Usage: Please note that reconstituted probe mix should be subjected to a minimum number of freeze-thaw cycles. For daily and short-term use, the mix can be stored at +2 to +8 °C in the dark for up to a month. For long-term use: We recommend freezing reconstituted probes in the dark at -15 to -30 °C for storage lasting longer than a month. Protocols: Detailed protocols by sample type can be found here: https://www.biosearchtech.com/stellarisprotocols. Storage and Handling: Shipping Conditions: Ambient; Storage Conditions: +2 to +8 °C
货号 名称 单位 购买
SMF-2026-1 Stellaris原位杂交探针,Quasar® 570标记的人GAPDH探针 1 nmol 商城订购

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