详细描述:
MiracleHyb hybridization solution was developed for use in Northern analysis when sensitive detection of low-abundance messages is a priority. In typical Northern applications, high complexity RNA samples are sizefractionated by agarose gel electrophoresis, then the resolved RNAs are transferred by blotting to a solid support. The immobilized RNAs may then be interrogated with one or more gene-specific probes. MiracleHyb is useful for hybridization experiments to detect both low- and high-abundance RNAs. When detecting low-abundance transcripts or when high signal intensity is required, a 24-hour hybridization is performed to achieve maximum sensitivity without increasing background levels. When studying high-abundance genes, or when quick results are important, hybridization time may be reduced to 1–2 hours. MiracleHyb can also be used in Southern and slot-blot formats.