详细描述：
Pfu DNA ligase is a recombinant enzyme derived from Pyrococcus furiosushyperthermophilic marine archaebacterium that catalyzes linkage of adjacent 5'-phosphate and 3'-hydroxy ends of double-stranded DNA at 45° to 80°C, with a temperature optimum near 70°C for nick-sealing reactions. It has higher ligation specificity and lower background than Tth DNA ligase.
