详细描述：
The RNAMaxx transcription kit uses T7 RNA polymerase to synthesize RNA from a double-stranded DNA template containing the T7 promoter. To generate high RNA yields, high concentrations of ribonucleotides are added to the reaction mixture. When a ribonucleotide is incorporated into RNA, a molecule of pyrophosphate is released. High concentrations of pyrophosphate have been shown to inhibit further polymerization. To remove this inhibition, yeast inorganic pyrophosphatase has been added to the reaction.1 The use of a higher concentration of ribonucleotides and the addition of yeast inorganic pyrophosphatase in the reaction mixture increases the RNA yield 10 to 30 times the yield produced by traditional in vitro transcription methods.
