详细描述：
EnGen® Lba Cas12a (Cpf1) from Lachnospiraceae bacterium ND2006 is a programmable DNA endonuclease guided by a single 41-44 nucleotide guide RNA (gRNA) (1). Targeting requires a gRNA complementary to the target site as well as a 5’ TTTN protospacer adjacent motif (PAM) on the DNA strand opposite the target sequence. Cleavage by EnGen Lba Cas12a (Cpf1) occurs ~18 bases 3’ of the PAM and leaves 5’ overhanging ends. EnGen Lba Cas12a (Cpf1) has Simian virus 40 (SV40) T antigen nuclear localization sequences (NLS) at both the N and C-termini of the protein.
来源：EnGen Lba Cas12a from Lachnospiraceae bacterium ND2006 is expressed as a N-terminal 6XHis-tagged fusion in E. coli.
储存温度：-20°C
储存缓冲液：0.1 mM EDTA ；50% Glycerol ；500 mM NaCl ；20 mM sodium acetate ；0.1 mM TCEP ；pH 6.0 @ 25°C
热失活条件：65°C for 10 min
