NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina®, 96 Rxns
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详细描述:
The NEBNext® Single Cell/Low Input RNA Library Prep Kit for Illumina® uses a template switching method to generate full length cDNAs directly from single cells or 2 pg – 200 ng RNA, followed by conversion to sequence-ready libraries using the Ultra™ II FS workflow. This unique workflow enables generation of the highest yields from a broad range of inputs, and superior transcript detection, while providing reliably consistent performance.
试剂盒成分:NEBNext® Single Cell RT Primer Mix、NEBNext® Single Cell RT Buffer、NEBNext® Template Switching Oligo、NEBNext® Single Cell RT Enzyme Mix、NEBNext® Single Cell cDNA PCR Master Mix、NEBNext® Single Cell cDNA PCR Primer、NEBNext® Cell Lysis Buffer、Murine RNase Inhibitor、NEBNext® Bead Reconstitution Buffer、NEBNext® Adaptor Dilution Buffer、TE Buffer、Nuclease-free Water、NEBNext® Ligation Enhancer、NEBNext® Ultra™ II Ligation Master Mix、NEBNext® Ultra II Q5® Master Mix、NEBNext® Ultra™ II FS Enzyme Mix、NEBNext® Ultra™ II FS Reaction Buffer
储存条件:-20°C