详细描述:
Hi-T7 RNA Polymerase is an engineered DNA-dependent RNA polymerase that is highly specific for T7 phage promoters and is designed to function at higher temperatures than the wild-type bacteriophage T7 RNA Polymerase. Hi-T7 RNA Polymerase functions at an optimal temperature of 50-52°C.
来源:An E. coli strain that carries a plasmid encoding the engineered T7 RNA Polymerase gene.
反应条件:1X Hi-T7™ RNA Polymerase Reaction Buffer Supplement with 0.5 mM ATP, 0.5 mM GTP, 0.5 mM UTP and 0.5 mM CTP 。Incubate at 50°C
储存温度:-20°C