详细描述:
Thermolabile Exonuclease I catalyzes the removal of nucleotides from single-stranded DNA (ssDNA) in the 3′ to 5′ direction at 37°C, and can be easily heat-inactivated at 80°C for 1 minute. This enzyme has no activity on linear/circular dsDNA or RNA. Thermolabile Exonuclease I is ideal for:
Removal of single-stranded primers in PCR reactions prior to DNA sequencing or SNP analysis
Removal of single-stranded primers for nested PCR reactions
Removal of single-stranded DNA from dsDNA
来源:An E. coli strain that carries a cloned Thermolabile Exonuclease I gene originating from a psychrophilic marine bacterium.
单位定义:One unit of Thermolabile Exonuclease I is defined as the amount of enzyme that will catalyze the release of 2 nmol of acid-soluble nucleotide in a total reaction volume of 100 μl in 6 minutes at 37°C in 50 mM Tris-HCl (pH 7.9), 100 mM NaCl, 10 mM MgCl2 and 100 μg/ml BSA with 0.17 mg/ml single-stranded [3H]-E.coli DNA.
反应条件:1X NEBuffer™ 3.1 ,Incubate at 37°C
热失活:80°C for 1 min
储存温度:-20°C