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核酸酶P1

Nuclease P 10,000 units

产品概述 技术指标 订购信息 服务支持
详细描述:
Nuclease P1 (from P. citrinum) is a zinc-dependent single-strand specific nuclease which hydrolyzes 3′->5′ phosphodiester bonds in RNA and ssDNA with no base specificity. Nuclease P1 also exhibits 3′-phosphomonoesterase activity (1). Although a single-strand specific nuclease (ssDNA and RNA-specific), Nuclease P1 does display some activity toward dsDNA in Nuclease P1 Reaction Buffer. If preferentially degrading single-stranded nucleic acids (ssDNA or RNA) in the presence of double stranded DNA (dsDNA), we recommend using Nuclease P1 in 1X NEBuffer 1.1 to limit activity on dsDNA while maintaining single-strand nuclease activity.
来源:Isolated from Penicillium citrinum. 单位定义:One unit is defined as the amount of enzyme required to liberate 1.0 µg of acid soluble nucleotides from Torula Yeast total RNA per min at 37°C in 1X Nuclease P1 Reaction Buffer. 反应条件:1X Nuclease P1 Reaction Buffer ,Incubate at 37°C 热失活:75°C for 10 min 储存温度:-20°C
货号 名称 单位 购买
M0660S 核酸酶P1 10,000 units 商城订购

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