详细描述：
PromoCell’s Primary Cancer Culture System is a standardized, defined and animal component-free (D-ACF) system for the isolation and culture of human primary tumor cells. The medium was designed to be the first universally applicable in vitro cell culture solution for the isolation and long-term culture of primary human malignancies, e.g. from patient tumor samples or patient-derived xenografts (PDX).
Due to precise stromal control, prolonged culture allows for functional selection of malignant cells. This principle enables access to an enriched population of primary cancer cells. The selection process dispenses with the use of toxic agents. Since on the cellular level malignancy itself is the only selection criterion, the cell diversity of the cancerous subpopulations of the original tumor is preserved.
Key features:
Perform up to five primary tumor isolations with one medium kit
Successfully prevent stroma overgrowth when isolating fresh tumor samples
Establish a culture of pure malignant cells within 4-6 weeks
Culture PDX tumor models in vitro
Defined and animal component-free formulation
Components:
Primary Cancer Cell Medium D-ACF, 250ml
SupplementMix for 250ml
NCCD surface treatment reagent, 2ml
