目标分子:His标记的蛋白质,含有组氨酸半胱氨酸和/或色氨酸氨基酸侧链的蛋白质;填料:WorkBeads 40 Zn-IDA;基质:刚性,高度交联琼脂糖;平均粒径(Dv50):45 µm;螯合配体:IDA;金属离子:Zn2+(Zink);静态结合载量:70 mg His-tagged protein/ml resin;动态结合载量(DBC): 50 mg His-tagged protein/ml resin ( The binding capacity is determined using a BabyBio Ni-IDA 1 ml. The binding capacity is dependent on the size of the target protein, and on the competition with impurities.);柱体积:1mL;柱尺寸:7 x 28 mm ;推荐流速:1 ml/min (150 cm/h);最大流速:5 ml/min (780 cm/h);最大背压:0.3 MPa, 3 bar, 43 psi ;化学稳定性:与用于蛋白质纯化的所有标准水性缓冲液,8 M尿素,6 M盐酸胍,非离子型去污剂,20%乙醇兼容。螯合物质(例如EDTA)会剥离金属离子;pH 稳定性:7-9(工作范围)、2-12(清洗);保存:2to25°C。