详细描述:
biotechrabbit™ GenUP Plasmid Kit is designed for fast and efficient plasmid purification from 0.5–15 ml bacterial suspensions in a mini format. After performing an alkaline lysis step and precipitating chromosomal DNA and bacterial proteins, plasmid DNA is bound to a Mini Filter, washed and then eluted in a low-salt buffer. Typically, yields are up to 60 µg plasmid DNA from a 15 ml bacterial suspension, depending on a plasmid copy number.
The purified plasmid DNA is ready to be used in all demanding molecular biology applications, including enzymatic reactions and sequencing.
特点:1、Universal kit for both high- and low-copy-number plasmids isolation;2、Fast and simple procedure;3、High yields of pure plasmid DNA suitable for all applications
应用:Plasmid DNA isolation from Gram-negative and Gram-positive bacteria
试剂盒成分:Solutions, Mini Filters and Vials
样品量: 0.5–15 ml菌液
抽提时间:约15分钟
结合力:约80 μg质粒DNA
得率:Variable: 45–60 µg high-copy plasmid DNA is typically purified from 15 ml culture, or 6–20 µg from 2 ml culture
纯度:A260/A280 1.7–2.0