详细描述:
biotechrabbit™ T4 DNA Ligase Rapid is an exceptionally pure, highly concentrated ligase for applications in which high enzyme concentrations are required. It is especially recommended for fast ligations.
T4 DNA Ligase Rapid is supplied with 5× Rapid Ligation Buffer containing PEG for fast 5–10 minutes ligation, ligation of low-concentration or blunt-end DNA.
T4 DNA Ligase catalyzes the formation of a phosphodiester bond between the terminal 5' phosphate and the 3' hydroxyl groups of duplex DNA or RNA. The enzyme efficiently joins blunt and cohesive ends and repairs single stranded nicks in duplex DNA, RNA or DNA–RNA hybrids.
特点:1、Exceptionally pure high-concentration T4 DNA Ligase;2、Perfect for rapid ligation
应用:Blunt and sticky end DNA ligation for cloning;Joining of linkers or adaptors to double-stranded DNA;Self-circularization of linear DNA
试剂盒成分:T4 DNA Ligase Rapid、5× Rapid Ligation Buffer
储存条件:-20°C