详细描述:
磷酸化c-Kit(Tyr719)的抗体能检测内源性酪氨酸719位点被磷酸化c-Kit蛋白的表达水平。本抗体能与酪氨酸磷酸化的PDGF受体发生交叉反应。多克隆抗体通过用多肽免疫动物得到,该磷酸化多肽是根据小鼠的c-Kit蛋白Tyr719附近的氨基酸序列合成的。抗体经过protein A和亲和层析纯化。Phospho-c-Kit (Tyr719) Antibody detects endogenous levels of c-Kit only when phosphorylated at tyrosine 719. The antibody cross-reacts with tyrosine-phosphorylated PDGF receptor.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Tyr719 of mouse c-Kit. Antibodies are purified by protein A and peptide affinity chromatography.