详细描述:
Acetyl-Histone H3 (Lys56) Antibody能够检测仅在Lys56位点乙酰化的内源性histone H3的蛋白水平。该抗体不能与在lysines 9、14、18或27位点乙酰化的histone H3发生交叉反应。通过合成的仅在Lys56位点乙酰化的人源histone H3蛋白氨基端相应的多肽片段去免疫动物从而制备出此多克隆抗体。通过蛋白A和多肽亲和层析纯化获得。Acetyl-Histone H3 (Lys56) Antibody detects endogenous levels of histone H3 only when acetylated on Lys56. This antibody does not cross-react with histone H3 acetylated on lysines 9, 14, 18 or 27.Polyclonal antibodies are produced by immunizing animals with a synthetic peptide corresponding to the amino terminus of histone H3 in which Lys56 is acetylated. Antibodies are purified by protein A and peptide affinity chromatography.