详细描述:
Phospho-A-Raf (Ser299) 兔多抗能检测内源性丝氨酸(229位点)磷酸化的A-Raf蛋白水平。这种抗体在生理条件下不会与c-Raf 和 B-Raf蛋白发生交叉反应。该多克隆抗体通过使用合成的与人源A-Raf蛋白丝氨酸(229位点)周围残基相一致的磷酸化肽段免疫动物而获得。该抗体经蛋白A和肽亲和层析纯化。Phospho-A-Raf (Ser299) Antibody detects endogenous levels of A-Raf only when phosphorylated at serine 299. The antibody does not cross-react with B-Raf or Raf-1 under physiological conditions.Polyclonal antibodies are produced by immunizing animals with a synthetic phosphopeptide corresponding to residues surrounding Ser299 of human A-Raf. Antibodies are purified by protein A and peptide affinity chromatography.